Chitosan에 依한 Mutans streptococci의 增殖抑制 및 saliva-coated hydroxyapatite beads에의 附着 抑制效果
Effects of chitosan on the growth and adsorption to saliva-coated hydroxyapatite beads of Mutans streptococci
소속 상세정보
이정환/Jung Hwan lee
김환규/장기완/이정환/김환규/장기완/Hwan Gyu Kim/Kee Wan Chang/Jung Hwan lee/Hwan Gyu Kim/Kee Wan Chang
KMID : 0355419980220040275
Abstract
결론
본 연구에서 는 chitosan에 의한 mutans streptococci 성장억제 효과와 S-HA beads에 대
한 부착억제 효과를 관찰하여 다음과 같은 결론을 얻었다.
1. Chitosan농도에 따른 S. mutans KCTC 3065 균주의 성장억제 효과를 확인한 결과,
100㎍/㎖에서는 28%, 500㎍/㎖에서는 31% 1000㎍/㎖에서는 52%의 성장억제 효과가 나타나
서, 농도증가에 따라 성장억제 효과가 농도의존적으로 증가되었다(p<0.05).
2. S. mutans KCTC 3065, S. cricetus KCTC 3292 및 S. rattus KCTC 3294 균주에서는
sucrose의 첨가시 14-17% 성장이 촉진되었으나, 통계적 유의성은 인정되지 않았다(p>0.05).
그러나, 이들 균주에 chitosan을 병용할 경우에는 42-47%의 성장억제 효과를 나타냈다
(p<0.05).
3. Chitosan의 S-HA beads에 대한 부착억제 실험 결과, S. mutans KCTC 3065 균주는
chitosan 첨가에 의해 68-72%의 부착억제 효과를 나타냈다.
#초록#
Chitosan, a chitin derivative by N-deacetylation, stimulates the ordered regeneration of
oral soft tissues, prevents the harmful action of organic acid, and exhibits bactericidal
action against several pathogens. In this study, we examined the effects of chitosan on
the growth and adsorption to saliva-coated hydroxyapatite beads of mutans streptococci
in vitro. The strains of mutants streptococci were S. mutans KCTC 3065. S. cricetus
KCTC 3292, S. rattus KCTC 3294, and S. sobrinus SL-1 KCTC 3308 strain. We found
that chitosan of sublethal concentrations had inhibitory effects on growth and adherence
to saliva-coated hydroxyapatite beads of mutans streptococci. The results obtained were
as follows.
1. The inhibitory effects of chitosan on the growth of S. mutans KCTC 3065 strain
were 28%, 31%, 52% at the concentrations of 100㎍/㎖, 500㎍/㎖, and 1000㎍/㎖ of
chitosan, respectively.
2. The rate growth inhibition was 20-28% by chitosan alone, and 42-58% by chitosan
with the 3 kinds of sugars supplemented as the substrates. The growth patterns in S.
rattus KCTC 3294 strain was differed from that of S. mutans KCTC 3065. The rate of
growth inhibition in S. mutans KCTC 3065 strain was 28% when chitosan was
supplemented.
3. The effects of with and without chitosan for the adherences to saliva-coated
hydroxyapatite were evaluated when the sucrose, glucose, and sorbitol were
supplemented as the substrates. The reduction of adsorption to saliva-coated
hydroxyapatite beads in 5. mutant KCTC 3065 strain was ranged from 47 to 58% when
sugar was supplemented.
키워드
부착억제효과; 성장억제효과; Chitosan; Adsorption inhibition; Chitosan; Growth inhibition;
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